C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori.
Identifieur interne : 000203 ( Main/Exploration ); précédent : 000202; suivant : 000204C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori.
Auteurs : Tao Geng [République populaire de Chine] ; Fuping Lu [République populaire de Chine] ; Huazhou Wu [République populaire de Chine] ; Yongsong Wang [République populaire de Chine] ; Dezhao Lou [République populaire de Chine] ; Nana Tu [République populaire de Chine] ; Feng Zhu [République populaire de Chine] ; Shuchang Wang [République populaire de Chine]Source :
- Journal of invertebrate pathology [ 1096-0805 ] ; 2020.
Abstract
The Janus kinase/signal transducer and activator of transcription cascade transduction (JAK/STAT) signaling pathway is highly conserved in mammals, but the pattern recognition receptors (PRRs) and their functions are unclear. We found that the expression pattern of Bombyx mori C-type lectin 5 (BmCTL 5) had a synergy relevance with the JAK/STAT signaling pathway against Beauveria bassiana. An RNAi assay, subcellular localization analysis, yeast two-hybrid technique, protein recruitment experiment and pathogen infection tests were used to explore the roles of BmCTL 5 in the JAK/STAT signaling pathway. Knock-down of the BmCTL 5 suppressed the JAK/STAT signaling pathway and the PO cascade of nodule melanization. BmCTL 5 is located in the cytomembrane and interacted with BmHOP both in yeast and B. mori ovary cells N (BmN cells). BmCTL 5 and the JAK/STAT signaling pathway was activated by B. bassiana but only slightly activated by B. mori cytoplasmic polyhedrosis virus (BmCPV), Nosema bombycis and bacteria LPS. These findings suggest that BmCTL 5 might be an important PRR for the JAK/STAT signaling pathway and may mediate the nodule melanization for fungi infection. These data provide insights into the immune mechanism of the JAK/STAT signaling pathway in insects and aid understanding of the mechanism of the JAK/STAT signaling pathway and adaptive immune systems in mammals.
DOI: 10.1016/j.jip.2020.107473
PubMed: 32946913
Affiliations:
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Electronic address: zf200537@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Zaozhuang University, Zaozhuang, 277160</wicri:regionArea><wicri:noRegion>277160</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Shuchang" sort="Wang, Shuchang" uniqKey="Wang S" first="Shuchang" last="Wang">Shuchang Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address: wangshuchang2001@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101</wicri:regionArea><wicri:noRegion>Haikou 571101</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of invertebrate pathology</title><idno type="eISSN">1096-0805</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Janus kinase/signal transducer and activator of transcription cascade transduction (JAK/STAT) signaling pathway is highly conserved in mammals, but the pattern recognition receptors (PRRs) and their functions are unclear. We found that the expression pattern of Bombyx mori C-type lectin 5 (BmCTL 5) had a synergy relevance with the JAK/STAT signaling pathway against Beauveria bassiana. An RNAi assay, subcellular localization analysis, yeast two-hybrid technique, protein recruitment experiment and pathogen infection tests were used to explore the roles of BmCTL 5 in the JAK/STAT signaling pathway. Knock-down of the BmCTL 5 suppressed the JAK/STAT signaling pathway and the PO cascade of nodule melanization. BmCTL 5 is located in the cytomembrane and interacted with BmHOP both in yeast and B. mori ovary cells N (BmN cells). BmCTL 5 and the JAK/STAT signaling pathway was activated by B. bassiana but only slightly activated by B. mori cytoplasmic polyhedrosis virus (BmCPV), Nosema bombycis and bacteria LPS. These findings suggest that BmCTL 5 might be an important PRR for the JAK/STAT signaling pathway and may mediate the nodule melanization for fungi infection. These data provide insights into the immune mechanism of the JAK/STAT signaling pathway in insects and aid understanding of the mechanism of the JAK/STAT signaling pathway and adaptive immune systems in mammals.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32946913</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0805</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of invertebrate pathology</Title><ISOAbbreviation>J Invertebr Pathol</ISOAbbreviation></Journal><ArticleTitle>C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori.</ArticleTitle><Pagination><MedlinePgn>107473</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0022-2011(20)30179-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jip.2020.107473</ELocationID><Abstract><AbstractText>The Janus kinase/signal transducer and activator of transcription cascade transduction (JAK/STAT) signaling pathway is highly conserved in mammals, but the pattern recognition receptors (PRRs) and their functions are unclear. We found that the expression pattern of Bombyx mori C-type lectin 5 (BmCTL 5) had a synergy relevance with the JAK/STAT signaling pathway against Beauveria bassiana. An RNAi assay, subcellular localization analysis, yeast two-hybrid technique, protein recruitment experiment and pathogen infection tests were used to explore the roles of BmCTL 5 in the JAK/STAT signaling pathway. Knock-down of the BmCTL 5 suppressed the JAK/STAT signaling pathway and the PO cascade of nodule melanization. BmCTL 5 is located in the cytomembrane and interacted with BmHOP both in yeast and B. mori ovary cells N (BmN cells). BmCTL 5 and the JAK/STAT signaling pathway was activated by B. bassiana but only slightly activated by B. mori cytoplasmic polyhedrosis virus (BmCPV), Nosema bombycis and bacteria LPS. These findings suggest that BmCTL 5 might be an important PRR for the JAK/STAT signaling pathway and may mediate the nodule melanization for fungi infection. These data provide insights into the immune mechanism of the JAK/STAT signaling pathway in insects and aid understanding of the mechanism of the JAK/STAT signaling pathway and adaptive immune systems in mammals.</AbstractText><CopyrightInformation>Copyright © 2020. Published by Elsevier Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Geng</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Fuping</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Huazhou</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yongsong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, Hainan University, Haikou 570228, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lou</LastName><ForeName>Dezhao</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Hainan University, Haikou 570228, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tu</LastName><ForeName>Nana</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>College of Tropical crop, Hainan University, Haikou 570228, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, China. Electronic address: zf200537@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shuchang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address: wangshuchang2001@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Invertebr Pathol</MedlineTA><NlmUniqueID>0014067</NlmUniqueID><ISSNLinking>0022-2011</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Beauveria bassiana</Keyword><Keyword MajorTopicYN="N">Bombyx mori</Keyword><Keyword MajorTopicYN="N">C-type lectin 5</Keyword><Keyword MajorTopicYN="N">JAK/STAT signaling pathway</Keyword><Keyword MajorTopicYN="N">Pattern recognition receptors</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>18</Day><Hour>20</Hour><Minute>13</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32946913</ArticleId><ArticleId IdType="pii">S0022-2011(20)30179-8</ArticleId><ArticleId IdType="doi">10.1016/j.jip.2020.107473</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Geng, Tao" sort="Geng, Tao" uniqKey="Geng T" first="Tao" last="Geng">Tao Geng</name></noRegion><name sortKey="Lou, Dezhao" sort="Lou, Dezhao" uniqKey="Lou D" first="Dezhao" last="Lou">Dezhao Lou</name><name sortKey="Lu, Fuping" sort="Lu, Fuping" uniqKey="Lu F" first="Fuping" last="Lu">Fuping Lu</name><name sortKey="Tu, Nana" sort="Tu, Nana" uniqKey="Tu N" first="Nana" last="Tu">Nana Tu</name><name sortKey="Wang, Shuchang" sort="Wang, Shuchang" uniqKey="Wang S" first="Shuchang" last="Wang">Shuchang Wang</name><name sortKey="Wang, Yongsong" sort="Wang, Yongsong" uniqKey="Wang Y" first="Yongsong" last="Wang">Yongsong Wang</name><name sortKey="Wu, Huazhou" sort="Wu, Huazhou" uniqKey="Wu H" first="Huazhou" last="Wu">Huazhou Wu</name><name sortKey="Zhu, Feng" sort="Zhu, Feng" uniqKey="Zhu F" first="Feng" last="Zhu">Feng Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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